Unlike most creatures, Colossal Amoebae spawn around the player instead of on a specific spawning tile.
While the player is inside the Passage, the game will attempt to spawn a Colossal Amoeba every 8–10 seconds. If there are more than two Colossal Amoebae within a 50 tile radius of the player, spawning will be placed on cooldown for 2–5 minutes. Additionally, they cannot spawn if Urschleim is nearby.
Colossal Amoebae will automatically despawn if there is no player within a 50 tile radius.